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Evolution of the Ca2+ conduction mechanism with increasing absolute value of effective fixed charge |Qf|, showing the Coulomb blockade oscillations of multi-ion conduction/blockade states. The neutralized states Zn providing blockade are interleaved with resonant conduction states Mn. The |Qf| value increases from top to bottom, as shown. Green circles indicate Ca2+ ions, unfilled circles show vacancies (virtual empty states during the knock-on process). The right-hand column indicates the preliminary identifications of particular channels/mutants corresponding to particular mechanisms. Mutants studied here are shown in red.
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