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Table 1

Main properties of the wild type (LESWAS) and mutant (LASWAS and LEDWAS) NaChBac bacterial channels generated and used for the present patch-clamp study. Here Qf stands for the nominal fixed charge at the selectivity filter and IC50 is the [Ca] threshold value providing 50% blockade of the Na+ current. Qualitative properties (selectivity and AMFE) are marked as “+” where present and “−” where absent.

Channel SF amino acid sequence Nominal Qf/e Ca2+/Na selectivity Divalent blockade, AMFE
Zero-charge mutant LASWAS 0
NaChBac wild-type LESWAS −4 + (Na > Ca)
Added-charge mutant LEDWAS −8 + (Ca > Na) + (IC50 = 5 μM)

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