Main properties of the wild type (LESWAS) and mutant (LASWAS and LEDWAS) NaChBac bacterial channels generated and used for the present patch-clamp study. Here Qf stands for the nominal fixed charge at the selectivity filter and IC50 is the [Ca] threshold value providing 50% blockade of the Na+ current. Qualitative properties (selectivity and AMFE) are marked as “+” where present and “−” where absent.
|Channel||SF amino acid sequence||Nominal Qf/e||Ca2+/Na selectivity||Divalent blockade, AMFE|
|NaChBac wild-type||LESWAS||−4||+ (Na > Ca)||–|
|Added-charge mutant||LEDWAS||−8||+ (Ca > Na)||+ (IC50 = 5 μM)|
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.